Characterization of Xylose Uptake in the Yeasts Pichia heedii and Pichia stipitis

Effect of Oxygenation on Xylose Fermentation by Pichia stipitis.

The effect of oxygen limitation on xylose fermentation by Pichia stipitis (CBS 6054) was investigated in continuous culture. The maximum specific ethanol productivity (0.20 g of ethanol g dry weight h) and ethanol yield (0.48 g/g) was reached at an oxygen transfer rate below 1 mmol/liter per h. In the studied range of oxygenation, the xylose reductase (EC 1.1.1.21) and xylitol dehydrogenase (EC...

Metabolic Network Analysis of Xylose Metabolism by Pichia Stipitis

The conversion of pentoses to ethanol is one of the major barriers of industrializing lignocellulosic ethanol processes. As the most promising native strain for pentoses fermentation, Pichia stipitis has been widely studied for its xylose fermentation. In spite of the abundant experimental evidence regarding ethanol and by-products production under various aeration conditions, the mathematical ...

Title A novel strictly NADPH-dependent Pichia stipitis xylose

Growth, death, and oxygen uptake kinetics of Pichia stipitis on xylose.

Pichia stipitis NRRL Y-7124 has potential application in the fermentation of xylose-rich waste streams, produced by wood hydrolysis. Kinetic models of cell growth, death, and oxygen uptake were investigated in batch and oxygen-limited continuous cultures fed a rich synthetic medium. Variables included rates of dilution (D) and oxygen transfer (K(1)a) and concentrations of xylose (X), ethanol (E...

Pichia stipitis genomics, transcriptomics, and gene clusters

Genome sequencing and subsequent global gene expression studies have advanced our understanding of the lignocellulose-fermenting yeast Pichia stipitis. These studies have provided an insight into its central carbon metabolism, and analysis of its genome has revealed numerous functional gene clusters and tandem repeats. Specialized physiological traits are often the result of several gene produc...